CAS9:CRISPR-CAS9系统的天然 噬菌体源“开关”
N. meningitidis:脑膜炎奈瑟菌
NmeCas9:脑膜炎奈瑟菌的Cas9,N为来源细菌的“属名”,me为来源细菌的“种名”。
结论:脑膜炎奈瑟菌Cas9体系中发现的噬菌体来源“可抑制CRISPR蛋白”,具有关闭CRISPR-Cas9系统的功能;可以改造为真核生物基因组编辑CRISPR-Cas9工具库的新工具。
These anti-CRISPR proteins now enable “off-switches” for CRISPR-Cas9 activity and provide a genetically encodable means to inhibit CRISPR-Cas9 genome editing in eukaryotes.
结果展示:
Some mobile elements in bacteria encode protein inhibitors of Cas9. Three families of anti-CRISPRs inhibit N. meningitidis Cas9 in vivo and in vitro. Anti-CRISPRs bind directly to NmeCas9.
本研究发现了一些游离的 编码Cas9酶的蛋白质抑制剂的基因元件。以及三个蛋白家族可抑制CRISPR系统中脑膜炎奈瑟菌来源Cas9蛋白的活性,体内或体外实验均证明可以直接结合Cas9并发挥效果。
These proteins are potent “off-switches” for NmeCas9 genome editing in human cells
这些蛋白质是针对NmeCas9蛋白强力的”开关“,CRISPR/Cas9基因组编辑技术在人类细胞的应用升级主要取决于:在细胞确定位置,特定时间段或条件性地抑制Cas9蛋白的功能。
CRISPR-CAS系统的分型总结:CRISPR-CAS9 为II型系统
不同类型的工作模式:
We posited that nature would also yield Cas9 inhibitors in response to the evolutionary arms race between bacteriophages and their hosts. Here, we report the discovery of three distinct families of anti-CRISPRs that specifically inhibit the CRISPR-Cas9 system of Neisseria meningitidis.
本研究断定:在噬菌体与宿主之间的进化竞赛过程中,自然界也会产生天然的Cas9抑制剂。本研究报道了三个不同的家族的“拮抗CRISPR体系”,这个家族的蛋白专门抑制脑膜炎奈瑟菌的CRISPR-Cas9系统。
We show that these proteins bind directly to N. meningitidis Cas9 (NmeCas9) and can be used as potent inhibitors of genome editing by this system in human cells.
研究表明:这些蛋白质绑定脑膜炎奈瑟菌直接Cas9(NmeCas9)和可以作为有效的抑制剂,在这个人类细胞基因组编辑系统中发挥功用。
These anti-CRISPR proteins now enable “off-switches” for CRISPR-Cas9 activity and provide a genetically encodable means to inhibit CRISPR-Cas9 genome editing in eukaryotes.
这些“拮抗CRISPR体系”的蛋白启动了“关闭”CRISPR-Cas9蛋白活性的能力,提供了一个针对真核生物基因组进行编辑的手段。
噬菌体针对不同类型CAS9系统的工作机制
材料来源:Cell 2016
原文:Naturally Occurring Off-Switches for CRISPR-Cas9
来源:赛晴生物
本文经授权发布,不代表TIMEDOO立场。如若转载请联系原作者。
满满的都是干货! 😉
@Damon chow: yes 😀
受益匪浅啊!良心之作,作者要吃多点干货啊
赛晴生物话说是哪里的公司?
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